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KMID : 0379520060220040315
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한국독성학회지
2006 Volume.22 No. 4 p.315 ~ p.322
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Cytocidal Effect of TALP-32 on Human Cervical Cancer Cell HeLa
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Park Ji-Hoon
Kim Jong-Seok
Yun Eun-Jin
Song Kyoung-Sub
Seo Kang-Sik
Kim Hoon
Jung Yeon-Joo
Yun Wan-Hee
Im Kyu
Hwang Byung-Doo
Park Jong-Il
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Abstract
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TALP-32 is highly basic protein with a molecular weight of 32 kDa purified from human term placenta. Some basic proteins such as defensins and cecropins are known to induce cell death by increasing membrane permeability and some of them are under development as an anticancer drug especially targeting multi-drug resistant cancers. Therefore, we investigated cytotoxic effect and mechanism of TALP-32 When HeLa cell was incubated with TALP-32, cytotoxicity was increased in time and dose dependent manner. As time goes by, HeLa cells became round and plasma membrane was ruptured. Increase of plasma membrane permeability was determined with LDH release assay. Also in transmission electron microscopy, typical morphology of necrotic cell death, such as cell swelling and intracellular organelle disruption was observed, but DNA fragmentation and caspase activation was not. And necrotic cell death was determined with Annexin V/Pl staining. The cytotoxicity of TALP-32 was minimal and decreased or RBC and Hep3B respectively. These data suggests that TALP-32 induces necrosis on rapidly growing cells but not on slowly growing cells implicating the possibility of its development of anticancer peptide drug.
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KEYWORD
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Necrosis, Peptide drug
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